Yeast Artificial Chromosome(YAC) ( YAC ) techniques play an important role in the analysis of human genome and the isolation and cloning of disease related genes. 人工酵母染色体(YAC)技术是人类基因组分析及疾病相关基因的分离、克隆中的关键技术。
Moreover, yeast artificial chromosome ( YAC ) cloning and site-specific mutation techniques can be combined with transgenic technique to investigate the developmental gene regulation of large genes ( clusters ) with mutations in different sites. 转基因动物技术与YAC(酵母人工染色体(YAC))克隆及定位突变技术的结合可望阐述大片段基因(簇)中不同部位对发育阶段特异性表达的调控规律。
Centromere, telomere and origins of replication are important elements to maintain the mitotic stability of chromosome. The Yeast Artificial Chromosome(YAC) ( YAC ) was firstly built in 1983 triumphantly by using these elements of yeast. 着丝粒、端粒和复制起点是保持染色体在有丝分裂时稳定性的重要组分,利用酵母染色体的这些组分,1983年人们首次成功地构建了酵母人工染色体(YAC)(YAC)。
Reconstruction of large genomic segment coding for human immunoglobulin κ chain by meiotic homologous recombination of yeast artificial chromosome 利用酵母人工染色体(YAC)(YAC)减数分裂同源重组构建人免疫球蛋白κ链基因组大片段
It have been reported that the 210 kb yeast artificial chromosome ( YAC ) including the human a - lactalbumin gene could direct the transgene to express in a position-independent manner, therefore is an excellent expression vector to produce transgenic animal bioreactors. 根据报道含完整人α-乳白蛋白基因的YAC载体能指导外源基因的乳腺特异性高效表达,是生产动物乳腺生物反应器的理想表达载体。