The primary strategies of gene targeting in yeast are transposon tagging and mutagen-esis, genome-wide gene deletions and transformation-associated recombination. 在酵母中进行基因打靶的策略主要是转座子标记的突变、基于PCR方法的基因删除和转化相关重组。
Materials and Methods Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library, with the green fluore - scent protein gene as the reporter gene in the transposon. 方法利用同源重组技术,通过转座子上的GFP基因,从酵母基因组文库中,检测发生突变的菌株,从而筛选出酵母突变株。