All the 9 isolates shared the common membrane anchor region and N-terminal signal peptide. 9株猪源分离株类M蛋白信号肽、C末端细胞锚定区的序列均高度同源;
Methods Insect cells at different growth phases and in different culture media were infected with recombinant baculovirus carrying EBV MA gene, where the membrane anchor sequence was deleted. 方法携有截去穿膜区序列的EBVMA基因的重组昆虫杆状病毒感染不同生长阶段及生长条件的昆虫细胞,应用免疫打点法及MasterImagineVDS影像摄录分析系统测定目的蛋白的表达量。
The secreted VP7 ( VP7s ) was further modified into a cell-surface-anchored antigen ( VP7tm ) by the addition of the membrane anchor sequence from the influenza virus HA. 通过在分泌型VP7基因下游融合流感病毒血凝素膜锚(MA)和胞质结构域编码序列,构建了表达后可锚定于宿主细胞膜表面的VP7基因。
Based on a non-linear FEM program of the membrane structure, a simple tensile membrane structure model was established and a series of parameter analyses were conducted to study the interactions between the membrane structure and its anchor foundation. 利用可以考虑边界位移影响和锚固基础共同作用的索膜结构非线性有限元程序,针对简单的张拉索膜结构模型,对张拉索膜结构与其锚固基础的共同作用进行了一系列分析。
Preliminary analysis of interactions between tensile membrane structure and its anchor foundation 张拉索膜结构与锚固基础共同作用初步分析