Methods Preliminary separation of endogenous anabolic androgenic steroids could be achieved using thin layer chromatography. The source of Testosterone and other endogenous anabolic androgenic steroids could be determined by their δ values with Gas Chromatography-Combustion-Isotope ratio mass spectrometry. 方法:采用薄层层析方法对尿样中内源性类固醇激素进行分离,再经气相色谱/燃烧炉/同位素比值质谱方法检测睾酮及其它内源性类固醇激素的δ值。