The highly focused laser beam was obtained by use of the objective with high numeric aperture, and the fluctuant fluorescence signals were collected by a sensitive single photon counting module. 采用高数值孔径(NA)物镜使激光束高度聚焦,并用同一物镜收集样品的荧光,单光子计数器用于实时记录荧光信号。
And the numeric simulation is done to get the multi-supporting results of the optical elements with large aperture. 针对各向同性圆形光学镜面做了具体的模拟计算,对圆形镜面的多点支承问题进行了初步研究。