The expressions of ubiquitin mRNA in the diaphragmatic muscle was determined with Northern blot analysis. 通过Northern杂交检测大鼠膈肌内泛素mRNA(UBQ)表达的变化;
Study on the changes in expression of ubiquitin mRNA in skeletal muscle in burned rats 烧伤早期大鼠骨骼肌组织泛素转录表达的变化研究
Two and six hours post-injury, contents of 3-methylhistidine in pectoralis major muscle, intercostal muscle and diaphragmatic muscle were determined with high performance liquid chromatography. The expressions of ubiquitin mRNA and C_2 subunit mRNA in those muscles were assessed with Northern blot analysis. 分别于伤后2h和6h,采用高效液相-荧光法检测胸大肌、肋间肌和膈肌内三甲基组氨酸(3-MH)的含量,Northern杂交检测泛素、蛋白酶体C2亚基mRNA表达的变化。