The contrast of telomerase activity between cytoplasmic and nuclear extracts of hepatocellular carcinoma samples 肝癌组织细胞质和细胞核中端粒酶活性比较
METHODS : Nuclear protein was extracted, p38 protein kinase in nuclear extracts was analyzed by Western blot. 方法:提取细胞核蛋白,采用Western印迹分析p38蛋白激酶水平。
The quality of nuclear extracts was satisfied the demand of TF microarray experiment through the quantitative detection. 2. 实验结果:1.所提取的细胞核蛋白经过定量检测符合芯片实验需求。
In vitro, the removal of Elongator complex from the HeLa nuclear extracts can decrease the rate of transcription of chromatin templates, which also provides biochemical evidence that human Elongator complex takes part in the transcription elongation. 在体外去除HeLa细胞抽提物中的Elongator复合物可降低其转录染色质模板的速率,也为人Elongator复合物参与转录延伸提供了生化证据。
Western blot analysis was used to assess the induction of Egr-1 protein in liver tissue, cytoplasm and nuclear extracts. 肝组织、胞浆及核提取物(NE)中Egr-1蛋白的表达。