In recent years, various CSCs were successfully isolated from different types of solid tumor by using technologies of fluorescence activated cell sorting ( FACS ) or immune-magnetic beads which based on expression of distinct markers of stem cells. 最近几年随着研究的深入,通过基于独特干细胞标志的表达检测的流式分选和免疫磁珠分选技术,科研工作者又陆续从不同类型的实体瘤中成功的分离出相应的肿瘤干细胞。
The effect of HDL on EPC proliferation was estimated on the 7th day of cell cultivation using MTT assay, confocal microscopy and fluorescence activated cell sorting. 在细胞分化的特定阶段,通过MTT、共聚焦显微镜检查和流式细胞术等方法,检测HDL对EPC增殖、抗原表达及细胞周期的影响。