Methods The transmission of Lucifer Yellow(LY) in cultural cells was observed by the methods of scrape-loading dye transfer. 方法用划痕标记染料示踪技术检测黄色荧光素在培养细胞中的传递情况。
Results : The apparent permeability coefficients ( Papp ) of Lucifer yellow and propranolol markers of the paracellular and transcellular respectively were all within the range of the reported ones. 结果细胞旁转运及跨细胞被动转运的标记物荧光黄和普奈洛尔的表观渗透系数(Papp)值均与文献报道一致;
METHODS : The techniques used in this study were brain slice patch clamping whole cell recording, lucifer yellow ( LY ) staining, immunofluorescence and laser scanning confocal microscopy ( LSCM ). 方法:采用脑片膜片钳全细胞记录、细胞内荧光黄染色、免疫荧光和激光共聚焦显微镜相结合的技术。
Methods Whole cell patch clamp recordings were made from RGCs in the acute retinal slices of Long Evans and Wistar rats aged postnatal 0-31 d. RGCs were stained with Lucifer Yellow(LY) diffused into cells during whole-cell recordings for the purpose of morphological study. 方法取出生后0~31dLongEvans和Wistar大鼠,各按睁眼时间分为两组,制备视网膜片,行膜片钳全细胞记录,同时行组织切片和细胞内染色,观察形态特征。
Lucifer yellow CH ( LY ) could pass through gap junction to neighboring cells, which were directly observed under inverted fluorescent microscope from different groups and at different hypoxia / reperfusion by scrape loading and dye transfer ( SLDT ). 采用细胞划痕技术,在倒置荧光显微镜下直观地观察不同组别、缺氧再灌注不同时间罗氏黄经过缝隙连接流通情况。