[ Methods ] () Experimental part : After being separated, cultured and identified, HUVEC was stimulated by ox-LDL, then MMP-2 level in supernatant of cultured HUVEC was determined by Enzyme Linked Immuno Sorbent Assay(ELISA) ( ELISA ). [方法](一)实验部分:分离、培养及鉴定HUVEC后,用ox-LDL刺激HUVEC,应用双夹心酶联免疫测定法(ELISA)测定培养上清液MMP-2水平;