Objective To establish RT-PCR method for Puumala virus(PUU) ( PUUV ) detection in order to confirm existence of Puumala virus(PUU) in Jilin province and analyze gene sequence of Puumala virus(PUU). 目的建立检测普马拉病毒(PUU)的RT-PCR方法,确认吉林省存在普马拉病毒(PUU),并对病毒的基因片段进行分析。