As one of the down stream signaling molecules of calcium, CaMK ⅱ facilitates the inactivation of maturation promoting factor ( MPF ) and cytostatic factor ( CSF ) following fertilization, as well as the spindle microtubule organization and centrosome duplication. CaMKⅡ作为Ca2+的下游信号分子,在受精后促进成熟促进因子(MPF)和细胞静止因子(CSF)的失活,并调节纺锤体微管的组装和中心体的复制过程。
MPF ( maturation of promoting factor ), which is consisted of catalytic domain P34 ~ ( cdc2 ) and regulative domain cyclin, plays a crucial role on the regulation of cell cycle. 成熟促进因子(maturationpromotingfactor,MPF)由催化亚单位P34cdc2和调节亚单位cyclin组成,对细胞周期的调控起着重要作用。
RESULTS : The best maturation promoting factor for DC was MCM. DC reached the climax on the 7th day. 结果:DC较好的促成熟因子(MPF)是MCM,第7日时达到高峰;