Prokaryotic Expression and Functional Analysis of Purine Nucleoside Phosphorylase(PNP) of the Key Enzyme for Ribavirin Produced 合成利巴韦林的关键酶嘌呤核苷磷酸化酶(PNP)的原核表达及活性分析
Purine nucleoside phosphorylase inhibitor screening using immobilized enzyme reactor by capillary electrophoresis 基于毛细管电泳在柱酶微反应器技术筛选嘌呤核苷磷酸化酶(PNP)抑制剂
Objective To construct three expression vectors harboring PNP ( purine nucleoside phosphorylase ) gene under three different promoters. 目的分别构建三种启动子调控的大肠杆菌嘌呤核苷磷酸化酶(PNP)(PNP)基因表达载体,检测、分析三者的表达差异。
The activity of purine nucleoside phosphorylase and release of purine were assayed by HPLC. 使用高效液相色谱技术测定嘌呤代谢产物(尿酸、黄嘌呤、次黄嘌呤、次黄苷)及嘌呤核苷磷酸化酶(PNP)的活性。
Effect of gamma irradiation on erythrocyte purine nucleoside phosphorylase activity and erythrocyte membrane lipid peroxidation in vitro and in vivo 离体与整体照射对大鼠红细胞嘌呤核苷磷酸化酶(PNP)活力与红细胞膜脂质过氧化值的影响