Conclusions This result indicate that PCR primer introduced V region N terminal changes may seriously affect the antigen binding activity. 结论在构建小分子抗体时,PCR引物引入的氨基酸残基的改变可影响所表达抗体的活性。
Antigen binding site of the polymorphism of MHC-I molecules can make a large number of antigenic peptide recognition. 抗原结合(AB)部位的多态性可使得MHC-I分子识别大量的抗原肽。
Objective Antigen binding fragments ( Fab ) were purified by affinity chromatography. 目的通过亲和层析的方法纯化基因工程人抗体Fab(Fragment,antigenbinding)片段。
Objective To control the quality of seed cells for production of therapeutic hepatocellular McAb HAb18.Methods The cell growth, antibody production rate and antigen binding activity were determined by cell counting, ELISA and FCM respectively. 目的进行治疗性肝癌单克隆抗体HAb18生产用种子细胞的质量控制研究。方法用细胞计数、ELISA和FCM分别检测细胞生长、抗体生成速度和抗原结合(AB)活性;
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed. 以该融合蛋白为固相抗原,对噬菌体抗体库进行3轮淘筛,并对所获阳性克隆进行抗原结合(AB)活性测定和DNA序列测定。