Improvement to the Method of Representational Difference Analysis(RDA) 对代表性差异分析(RDA)方法的改进
Both representational difference analysis ( RDA ) and the modified cDNA RDA are new techniques that have been developed in recent years. They could be applied broadly in analyzing the different expressing genes and screening different genes. 代表性差别分析方法(RDA)以及改进的cDNA代表性差别分析方法(cDNARDA)是近年发展起来的新技术,在基因差异表达分析和筛选差异基因的研究中有着广泛的应用前景。
Comparison of representational difference analysis of cDNA and suppression subtractive hybridization technology cDNA代表性差异分析(RDA)和抑制性减法杂交技术的比较
Methods We investigated the different gene expression within 1 hour after 2 / 3 partial hepatectomy by representational difference analysis and in primary cultured hepatocytes system. 方法利用表达性差异显示分析技术和序列分析研究2/3肝部分切除后1h及原代培养大鼠肝细胞体系的选择性基因表达。
Differential display technique is an important method to isolate differentially expressed gene. Comparing to other methods like representational difference analysis, suppression subtractive hybridization and serial analysis of gene expression, differential display technique is used in higher frequency. 差异显示技术是分离差异表达基因的重要方法与手段,同其他研究基因差异表达的方法如RDA,SSH,SAGE相比,差异显示技术是其中使用频率较高的方法。