This method, with the advantages of convenient, high-flux and cost efficient, can be used in screen transposon mutant library to find out the host factors in bacteria that can impact the excision efficiency of exogenous gene cassettes in integron. 该方法具有简便、高通量、费用低等优点,可运用以对转座子插入突变文库进行高通量筛选以寻找宿主菌中影响整合酶介导的基因盒剪切频率的因素。
RFLP ( restriction fragment length polymorphism ) analysis showed that the transposon is 3 cM ( centiMorgan ) away from the S locus and retains high transpositional activity with a germinal excision frequency of 20 %. 限制性酶切片段长度多态性(RFLP)的分析证明该转座子距S位点约3cM(centiMorgan),并且保持了较高的转位活性,其配子切除频率高达20%。