Methods Glomeruli and tubules of rat kidney were isolated by laser microdissection and pressure catapulting system. Gene expression level of transforming growth factor ( TGF - β 1 ) and fibronectin ( FN ) were assessed. 方法应用自动激光微分离方法分离大鼠肾脏的肾小球及肾小管,观察转化生长因子(TGFβ1)和纤维连接蛋白(FN)的基因表达变化。