Northern blot analyses revealed that ZNF474 mRNA was exclusively expressed in testis and ovary and has one transcript. 原位杂交显示ZNF474基因在正常成人睾丸组织各级生精细胞、隐睾组织以及精原细胞癌组织中均有较高表达。
The regulations in the transcription level are the main method of eukaryotic genes ' modulation, that effect and control many biological processes, and genes differently expressed between hybrids and parents may have relation to the transcript factors. 转录水平的调控是真核基因表达调控的主要方式,它影响和控制着许多生物学过程,杂交种和亲本间基因的差异表达可能与转录因子有关。
Objective To investigate the differentially expressed gene profile between high rate and low rate metastatic large cell lung cancer cell lines via differential display reverse transcript PCR ( DDRT PCR ). 目的通过研究人高、低转移肺大细胞癌细胞系基因差异表达谱,克隆与肿瘤转移相关的基因。
Results Survivin expressed in fresh CB CD 34 + cells, its protein level was 9.4 ± 1.2, faint signal of the mRNA transcript band was detected. 结果Survivin基因在新鲜脐血CD34+细胞中有表达,蛋白表达率为9.4±1.2,SURVIVINMRNA为弱阳性。