Methods : The frameworks of human antibody germline genes and semi-random CDR3s ( complementarity determining region 3 ) were synthesized artificially. The antibody genes were synthesized by SOE-PCR ( splicing overlap extension PCR ). 方法:选择部分使用频率较高的人抗体胚系基因家族的框架区基因进行人工合成,同时人工设计合成半随机CDR3,通过重叠拼接延伸PCR的方法合成抗体基因。
To observe the expression of TCR V β subfamily T cells, complementarity determining region 3 ( CDR3 ) of TCR β with 24 variable region genes was amplified in peripheral blood mononuclear cells from 12 B-CLL cases using RT-PCR. 利用RT-PCR扩增12例B-CLL外周血单个核细胞的TCRVβ基因24个亚家族的互补决定区(CDR)3(CDR3),以了解T细胞的表达情况。
Feature of immunoglobulin heavy chain complementarity determining region 3 gene in neonates with different gestational age 不同胎龄新生儿免疫球蛋白重链基因CDR3序列特征