Expressed Sequence Tag(EST) ( EST ) is important structural information of gene in the genome of organisms. 表达序列标签(ExpressedSequenceTag,EST)是生物基因组中最有价值的基因结构信息。
Gene cloning and expression analysis based on expressed sequence tag 基于表达序列标签(EST)的基因克隆和基因表达分析研究进展
In recent years, main methods of studying the plant functional genomics include expressed sequence tag, serial analysis of gene expression, DNA micro array, reverse genetics and so on. 近年来植物功能基因组学的研究技术主要包括表达序列标签、基因表达的系列分析、DNA微阵列和反向遗传学等。
The research metheds of functional genomics, mainly include Expressed Sequence Tag(EST) ( EST ), Serial Analysis of Gene Expression ( SAGE ), cDNA microarrays and DNA chips, Reverse genetics, Proteomics and Bioinformatics. 植物功能基因组学的研究方法主要包括:表达序列标签(EST)、基因表达序列分析(SAGE)c、DNA微阵列和DNA芯片、反求遗传学、蛋白质组学和生物信息学;
Four of the 24 clones were part of known human genes, 8 were known porcines expressed sequence tag ( EST ), and 9 held similar homology with known human genes. Three clones were not matched. 其中4个为已知的人的基因片段,8个为猪的表达序列标记(EST),9个片段与人的基因有高度的同源性,3个片段未检索到相匹配的同源性序列。