In this study, we assemble a positive feedback loop for the production of T7 RNA polymerase using basic molecular elements, including CMV promoter, T7 promoter and the internal ribosomal entry site ( IRES ). 在本课题中,利用基本的分子生物学元件,如CMV启动子、T7启动子、和内部核糖体进入位点(IRES)等,我们组装了一个T7RNA聚合酶的正反馈表达系统。
The polymerase chain reaction ( PCR ) machine is a basic instrument in molecule biology. 聚合酶链式反应仪(PCR仪)是分子生物学的基本仪器。
Polymerase chain reaction is a specific nucleic acid sequence amplification in vitro, which is one of basic work in the modern molecular biology experiments, playing an important role in the areas such as life sciences, genetics and medicine. 聚合酶链反应(POLYMERASECHAINREACTION,PCR)是一种体外特定核酸序列扩增技术,是现代分子生物学实验的工作基础之一,在生命科学、遗传学和医学等领域发挥着重要作用。