Peptide mass fingerprints of differential protein spot C4, M7 and M9 had low homology with database proteins, they were candidates of associated proteins with colorectal cancer genesis and hepatic metastasis. 差异蛋白点C4、M7和M9肽指纹谱与已知蛋白同源性低,为候选大肠癌发生和肝转移相关新蛋白。
PCR products of the library were cloned into pGEM-Teasy vectors and the cDNAs were sequenced and analyzed by nucleotide homology comparison against GenBank Database using the BLAST network service. 将文库的PCR产物克隆入pGEM-Teasy载体并进行测序后,应用BLAST网络服务对测得的序列在GenBank数据库中进行同源性比较。
8202-1,8202-2 and 8206 were up-regulated in gastric cancer and precancerous lesion, they had high homology with known sequences in GenBank database, but their functions were unknown. 在胃癌及胃癌前病变中高表达的8202-1、8202-2和8206均与已知序列同源,但功能未知。
The 49 TDFs were composed of 20 function-known genes, 8 genes of function-unknown genes and 21 novel genes that showed homology only to un-annotated genomic sequences or no match in the nucleotide database. 在49条TDFs中,有20个编码已知功能蛋白,8个编码未知功能蛋白,其余的21个与未注释的基因组序列相似,或者在核苷酸数据库无同源序列。
Results The MF 1 cDNA sequence had 100 % homology with cytochrome oxidase subunit 1 gene ( CO1 ) by searching Gene Bank database; 结果克隆测序及与Genebank同源性比较表明,MF1序列与细胞色素氧化酶亚基1基因(CO1)有100%同源性。