Cyclic voltammetry was used to characterize the ssDNA immobilization and hybridization with its complementary DNA(CDNA) sequences by using methylene blue as indicator. 在硬脂酸铝离子膜上进行DNA探针的固定和与目标基因的杂交。
Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA(CDNA) of dengue virus 2 RNA. 新近制备了大量纯化的pEH920DNA,该质粒DNA插入了登革病毒2型核酸片段的互补DNA(CDNA)。
The chicken growth hormone ( GH ) complementary DNA(CDNA) ( cDNA ) have been isolated from a cDNA library, which was constructed with meat-type chicken breed pituitary. 本研究从肉用品种鸡的垂体文库中克隆分离了鸡生长激素cDNA,并作了核苷酸序列测定。
Methods : ( 1 ) Reverse transcription-polymerase chain reaction ( RT-PCR ) was use to obtain the TR gene complementary DNA(CDNA) ( cDNA ). 方法:(1)进行逆转录-聚合酶链式反应(RTPCR),获得TR基因cDNA;
The general strategy for cloning chymosin gene is to synthesize complementary DNA(CDNA) ( cDNA ) by reverse transcription of mRNA fron which introns have been spliced out and then the cDNA is cloned and expressed in a suitable host. 克隆凝乳酶的一般策略是经过mRNA反转录合成cDNA,将内涵子拼接掉后把cDNA克隆在表达宿主中进行表达。