Because immunoglobulins are different in species or subspecies and M proteins are monoclonal immunoglobulin, whether the used reagents are suitable or not still remains to be studied. 因Ig存在不同种类、亚类,加之M蛋白是单克隆Ig,所用试剂对M蛋白的检测是否完全适用有待探讨;
The levels of calcium and phosphorus, alkaline phosphatase ( ALP ), immunoglobulins ( Ig G, Ig A, Ig M ), serum complements ( C 3, C 4 ), and leukocyte count in the blood of the two groups were examined before and after treatment. 两组在治疗前后分别取血,检测血钙磷、碱性磷酸酶、免疫球蛋白(IgG、IgA、IgM)、血清补体(C3、C4)及血白细胞计数。
Methods : M-component in serum and urine was determined using cellulose acetate membrane electrophoresis, immunofixation electrophoresis and quantification of immunoglobulins. Immunohistochemistry was performed to detect the expression of IgE and its light chain. 方法对1例骨髓瘤患者的血清尿液采用醋酸纤维膜电泳、免疫固定电泳和免疫球蛋白定量等方法鉴定M蛋白,采用直接和间接免疫酶标技术检测瘤组织IgE及轻链的表达。