Detection of Cucumber green mottle mosaic virus by real time Reverse Transcriptase Polymerase Chain Reaction(RTPCR) 实时荧光RT-PCR方法检测黄瓜绿斑驳花叶病毒
The osteogenic marker osteopontin ( OPN ) mRNA were examined by reverse transcriptase polymerase chain reaction ( RT-PCR ). 利用反转录聚合酶链反应(RT-PCR)技术在转录水平检测成骨细胞标记物骨桥蛋白(OPN)mRNA的表达。
The levels of RSV mRNA in different cells were tested by reverse transcriptase polymerase chain reaction ( RT-PCR ). 逆转录多聚酶联免疫(RT-PCR)法检测各细胞中病毒相对含量。
Three methods including the classical virus isolation and identification, reverse transcriptase polymerase chain reaction ( RT-PCR ) and indirect fluorescence assay ( IFA ) were used to detect avian influenza virus ( AIV ) in the sample and were compared. 对分别应用病毒分离鉴定、反转录-聚合酶链式反应(RT-PCR)、间接免疫荧光(IFA)3种方法对病料组织中的禽流感病毒(AIV)进行检测与比较研究。
Immunophenotyping of the blast cells was analyzed by flow cytometry with a panel of monoclonal antibodies. AML1 / ETO fusion gene was tested by nested reverse transcriptase polymerase chain reaction ( RT PCR ). 例2,应用流式细胞仪和一组单克隆抗体检测其白血病细胞的免疫表型,应用筑巢式逆转录聚合酶链反应(RTPCR)技术检测其AML1/ETO融合基因转录本。