Primers designed to achieve : high coverage, which can amplify all the target fragments; high specificity, that is, to other crops and there will be no amplification of biological gene caused by a possible false positive. 设计出的引物能达到:高覆盖度,即能扩增所有靶标片段;高特异性,即对其它作物和生物基因不会有扩增而造成可能的假阳性。
Moreover, contamination from biological cell or DNA and the inherent limitation of the latest molecular technology might give false positive and false negative test results. 同时也因为受到生物细胞或脱氧核糖核酸(DNA)污染及当前分子生物技术的局限,可能导致出现假阳性或假阴性的测试结果。