Results After antihemophilic factor antigen was tested by immunofluorescence test, The HUVEC cell line was identified. 结果经人第Ⅷ因子免疫荧光抗体检查法鉴定,脐带血管内皮细胞经原代培养后获得人脐静脉血管内皮细胞系。
Conclusion HUVEC cell line can be got from infant umbilical cord by primary cultivation, and antihemophilic factor antigen tested by immunofluorescence test is a good method for identification. 结论人脐静脉血管内皮细胞可从脐带获得并通过原代培养成为细胞系,而人第Ⅷ因子免疫荧光抗体检查法是鉴定人脐静脉血管内皮细胞的良好方法。
Objective To prepare stable standards for testing factor ⅷ coagulant activity ( F ⅷ∶ C ) using antihemophilic factor ( AHF ) fractionated from large pool plasma. 目的用超多人份混合血浆分馏抗血友病球蛋白(AHF)制备稳定的用于测定凝血因子促凝活性(F∶C)的标准品。